anti tgf β  (R&D Systems)

Journal: Communications Biology

Article Title: Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis

doi: 10.1038/s42003-025-08425-w

Figure Lengend Snippet: TNF-α tumor necrosis factor-α, IFN-ϒ interferon-ϒ, IL-23 interleukin 23, TGF-β1 transforming growth factor β1, TGF-β2 transforming growth factor β2. a En face atherosclerosis assay showing significantly less atherosclerosis in fortilin KO-MΦ-HC mice than in fortilin WT-MΦ-HC mice. b Cross-sectional atherosclerosis assay showing significantly less atherosclerosis in fortilin KO-MΦ-HC mice than in fortilin WT-MΦ-HC mice. Quantitative multiplex cytokine assays of ( c ) TNF-α, ( d ) IFN-ϒ, ( e ) IL-23, ( f ) TGFβ-1, and ( g ) TGF-β2. The scale bar in ( b ) equals 100 µm. Data are expressed as mean ± s.d., P values were determined by either two-sided Student’s t test ( a , b , f , g ) or two-tailed Mann–Whitney test ( c , d , e ).

Article Snippet: We first covalently coupled mouse α-TGF-β mAb (R&D Systems, Minneapolis, MN, USA; Clone 1D11, Catalog #: MAB1835-100) to tosylactivated magnetic beads (Dynabeads® M-280; Thermo Fisher, Catalog #: 14203) following manufacturer’s instructions.

Techniques: Multiplex Assay, Two Tailed Test, MANN-WHITNEY

Journal: Communications Biology

Article Title: Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis

doi: 10.1038/s42003-025-08425-w

Figure Lengend Snippet: CM conditioned media, WT THP1 WT-fortilin cells, KO THP1 KO-fortilin cells, oxLDL oxidized low density lipoprotein, UCDMSCs human umbilical cord derived mesenchymal stem cells, M media, F.C. fold change, α-TGF-β1-MB anti-TGF-β1-antibody-coated magnetic beads, ID-CM CM immunodepleted of TGF-β1, TGF-β1 ID media with TGF-β1 immunodepleted of TGF-β1. Time-course of RT-qPCR of RNA from UCDMSCs treated by either M only, M + TGF-β1, CM of THP1 KO-fortilin , or CM of THP1 WT-fortilin ( a ) for VSMC markers (αSMA ( b ); SM22α ( c ); and CNN1 ( d )), a differentiation marker (CD45; e ); and a marker of undifferentiated cells (CD105, f ) (n = 3 for each group). g ELISA of CM from THP1 WT-fortilin cells and THP1 KO-fortilin cells with and without oxLDL stimulation (n = 4 for each group). RT-qPCR of RNA from UCDMSCs treated by either M only with and without TGF-β1 ID, M + TGF-β1 with and without TGF-β1 ID, CM of THP1 KO-fortilin with and without TGF-β1 ID, or CM of THP1 WT-fortilin with and without TGF-β1 ID ( h ) for VSMC markers αSMA ( i ), SM22α ( j ), and CNN1 ( k ); a differentiation marker (CD45, l ); and a marker of undifferentiated cells (CD105, m ) (n = 3 for each group). Data are expressed as mean ± s.d. P values determined by one way ANOVA ( b – g , i – m ) are shown.

Article Snippet: We first covalently coupled mouse α-TGF-β mAb (R&D Systems, Minneapolis, MN, USA; Clone 1D11, Catalog #: MAB1835-100) to tosylactivated magnetic beads (Dynabeads® M-280; Thermo Fisher, Catalog #: 14203) following manufacturer’s instructions.

Techniques: Derivative Assay, Magnetic Beads, Quantitative RT-PCR, Marker, Enzyme-linked Immunosorbent Assay

Journal: Communications Biology

Article Title: Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis

doi: 10.1038/s42003-025-08425-w

Figure Lengend Snippet: FT fortilin, MΦ macrophages, VSMCs vascular smooth muscle cells, MSCs mesenchymal stem cells. The lack of fortilin in MΦ produces profound phenotypic changes, making MΦ anti-atherosclerotic. More specifically, fortilin deficiency in MΦ suppresses their proliferation and FC formation, but promotes their apoptosis, TGF-β1 secretion, and transdifferentiation into VSMCs, and also drives MSC differentiation into VSMCs via TGF-β1 ( b ), compared with normal fortilin expression ( a ).

Article Snippet: We first covalently coupled mouse α-TGF-β mAb (R&D Systems, Minneapolis, MN, USA; Clone 1D11, Catalog #: MAB1835-100) to tosylactivated magnetic beads (Dynabeads® M-280; Thermo Fisher, Catalog #: 14203) following manufacturer’s instructions.

Techniques: Expressing